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A modified tetramethylbenzidine method for measuring lipid hydroperoxides

โœ Scribed by Panakkezhum D. Thomas; Mark J. Poznansky

Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
528 KB
Volume
188
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A simple and sensitive spectrophotometric method for measuring lipid peroxides and peroxides in general is described. The method was developed by modifying an existing method based on the peroxidase activity of hemoglobin with tetramethylbenzidine as the electron donor. The modifications resulted in much improved sensitivity and reproducibility. With the modified method lipid peroxides as low as 2 nmol can be measured, a high sensitivity compared with other spectrophotometric methods. The absorbance is linear over a wide range of concentrations. It is suggested that this modified method in combination with the commonly used thiobarbituric acid method will give a better quantitation of lipid peroxidation.

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## Abstract Lipid oxidation causes deterioration of the quality of fatty fish during processing and storage. Its development should be evaluated by measurement of several oxidation products, among them the hydroperoxides. However, most of the methods for determining hydroperoxides already in use fa