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A modified protocol for in vitro maturation of mouse oocytes from secondary preantral follicles

✍ Scribed by Y. Tang; W. Wang; L. Ni; H.-C. Liu

Book ID
116479916
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
45 KB
Volume
96
Category
Article
ISSN
1556-5653

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## Abstract Oocytes were dissected from late, middle, and pre‐antral Graafian follicles from sexually mature mice (6–8 weeks old), cultured in vitro in a chemically defined medium for 18 hours, and scored for meiotic maturation. Between 83–91% of the oocytes removed from antral follicles (300–600 ΞΌ