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A modification of the Lowry method for detecting protein in media containing lignocellulosic substrates

✍ Scribed by L. U. L. Tan; M. K-H Chan; J. N. Saddler

Publisher
Springer Netherlands
Year
1984
Tongue
English
Weight
395 KB
Volume
6
Category
Article
ISSN
0141-5492

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✦ Synopsis

Clostridium thermocellum culture media containing crude cellulosic substrates, prepared by the steam-explosion of wood or straw, were found to interfere strongly with the Lowry method of protein determination.

Investigations into the mode of interferences demonstrated that either the intercept (blank value) and/or the slope (intensity of color development) were affected.

The efficiencies of precipitation procedures using the trichloroacetic acid method and the deoxycholate-trichloroacetic acid method were compared.

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