A modification of the Gibbard and Watkins method for determination of D-β-hydroxybutyrate
✍ Scribed by Vibeke Bitsch
- Book ID
- 115819346
- Publisher
- Elsevier Science
- Year
- 1972
- Tongue
- English
- Weight
- 117 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0009-8981
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📜 SIMILAR VOLUMES
A method is described for preparing the physiologically active isomer D(-)-phydroxybutyrate in quantities of several millimoles by the reduction of acetoacetate, catalysed by /3-hydroxybutyrate dehydrogenase at pH 8. Economy in the use of coenzyme is achieved by coupling the reaction to the oxidatio
The gas chromatographic method for the determination of poly-/3-hydroxybutyric acid (PHB) consists of a mild acid or alkaline methanolysis of poly-~-hydroxybutyric acid directly without previous extraction of PHB from the cells; this is followed by gas chromatography of the 3-hydroxybutyric acid met