A model for the role of HLA-DQ molecules in the pathogenesis of juvenile chronic arthritis

✍ Scribed by J. P. Haas; A. Andreas; B. Rutkowski; H. Brunner; E. Keller; J. Hoza; S. Havelka; G. Sierp; E. D. Albert

• Publisher: Springer
• Year: 1991
• Tongue: English
• Weight: 741 KB
• Volume: 11
• Category: Article
• ISSN: 0172-8172
• DOI: 10.1007/bf00332561

✦ Synopsis

Restriction fragment length polymorphism (RFLP) typing of MHC-class II loci DRB, DQA1, DQB1, DQA2 and DPB1 was performed in 94 patients with seronegative juvenile chronic arthritis (JCA) and 184 random controls. Analysis of allele frequencies and MHC-class II 4-loci haplotypes indicate: (1) Susceptibility to JCA is more strongly associated with the HLA-DQ subregion than with the HLA-DR subregion, especially in early onset pauciarticular JCA (EOPA-JCA). (2) Haplotype and sequence analysis show two independent MHC-class II associations for susceptibility to EOPA-JCA, one located in DQA1, the other in DPB1. (3) Two RFLP defined patterns of the DQA1 locus, DQA1.5 (DQA1*0501) and DQA1.8 (DQA1*0401, *0601) are strongly associated with the disease. (4) Analysis of amino-acid (AA) sequences coded in exon 2 of DQA1 reveals an AA sequence of six AAs common to all three associated DQA1 alleles. This suggests a model that includes a functional role for HLA-DQ molecules in the pathogenesis of JCA.