## Objective. The implied role of retroviruses in the pathogenesis of murine systemic lupus erythematosus (SLE) led us to study antiretroviral antibodies in a population-based SLE cohort. Methods. Immunoassays using whole virus and synthetic peptides were performed on sera from 72 patients with SL
A millipore filter assay for antibodies to native dna in sera of patients with systemic lupus erythematosus
β Scribed by Dr. Barry Ginsberg; Harold Keiser
- Publisher
- John Wiley and Sons
- Year
- 1973
- Tongue
- English
- Weight
- 606 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0004-3591
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Recently, a new radioimmunoassay-the polyethylene glycol (PEG) assay-was introduced to measure antibodies to double-stranded (ds) DNA. In this method, polyethylene glycol precipitation of formed 3H-DNMantiDNA complexes is used instead of the ammonium sulfate precipitation used in the Farr assay. In
A 96-well microplate ELISA for the detec-native E. coli DNA, calf thymus DNA, and tion of antibodies to DNA is described. A heat-denatured DNA. Using native E. coli number of buffers and precoating treat-DNA, virtually none of 35 normal sera had ments were used to evaluate the optimal any detectable
We examined the effect of DNase treatment of sera with antihistone activity. In non-systemic lupus erythematosus (SLE) sera, antihistone levels remained unmodified, but a significant decrease was observed in 7 of 11 SLE sera with anti-DNA antibodies. This was accompanied in some by an increase in an