A Micromethod of Estimating the Reactivity of Cellulose to Acetylation
β Scribed by R.Z. Pen; P.V. Mironov; O.A. Kolmakova
- Book ID
- 111556657
- Publisher
- SP MAIK Nauka/Interperiodica
- Year
- 2003
- Tongue
- English
- Weight
- 38 KB
- Volume
- 76
- Category
- Article
- ISSN
- 1070-4272
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π SIMILAR VOLUMES
A method for the determination of acetyl phosphate in cell-free extracts is described. The sensitivity of this method is 20 pmoYm1 of extract. An adaptation that permits the measurement of picomole quantities of acetyl CoA is also presented.
Modified cellulose nitrate membrane strips were applied in a new chromatographic procedure for rapid and sensitive estimation of adenosine deaminase (EC 3.5.4.4) and adenosine nucleosidase (EC 3.2.2.7). In this method the enzymes serve each other as reagents. The products of their subsequent action