A micromethod for estimation of adenosine deaminase and adenosine nucleosidase with modified cellulose nitrate membranes

✍ Scribed by Zenon Schneider

• Publisher: Elsevier Science
• Year: 1980
• Tongue: English
• Weight: 588 KB
• Volume: 108
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(80)90698-3

✦ Synopsis

Modified cellulose nitrate membrane strips were applied in a new chromatographic procedure for rapid and sensitive estimation of adenosine deaminase (EC 3.5.4.4) and adenosine nucleosidase (EC 3.2.2.7). In this method the enzymes serve each other as reagents. The products of their subsequent action are adenine and inosine, well separable on membrane strips, thanks to the different adsorptive affinities of these two compounds to the cellulose nitrate membranes. Employing adenine-labeled adenosine, microgram amounts of wet biological material may be used for estimation of the enzymes, The method has been applied to routine estimations of these two enzymes in various biological materials and examples are presented. A simple method is described for preparative purification and stabilization of adenosine nucleosidase of barley leaves used as reagent for adenosine deaminase assay.