The melting curve for a covalently closed circular DNA has been analyzed on the hasis of an expreasion for the supercoiling energy derived in terms of the elastic parameters of the macromolecule, treated as a homopolymer. The result obtained by applying the usual methods of statistical mechanics ind
A microfluidic study of mechanisms in the electrophoresis of supercoiled DNA
β Scribed by Dammika P. Manage; Iveta Imriskova-Sosova; D. Moira Glerum; Christopher J. Backhouse
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 389 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract We consider the problem of making allowance for superhelicity in the statisticalβmechanical calculations of fluctuational violations of the DNA double helix. A simple model is discussed, making it possible in the calculations to use an approach based on the theory of helixβcoil transiti
## Synopsis DNA supercoiling is both an interesting problem from the theoretical point of view and an important phenomenon affecting DNA functions in viuo. Experimentally, however, hardly more than the overall hydrodynamic shape, superhelical density, and enzymic or chemical reactivity of the para
## Abstract We have evaluated doubleβstranded DNA separations in microfluidic devices which were designed to couple a sample preconcentration step based on isotachophoresis (ITP) with a zone electrophoretic (ZE) separation step as a method to increase the concentration limit of detection in microfl