A semiempirical expression for the gel electrophoretic mobility of supercoiled DNA
β Scribed by Ansuman Lahiri; Srikanta Sen
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1992
- Tongue
- English
- Weight
- 324 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0006-3525
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## Abstract The electrophoretic migration of rigid rodlike DNA structures with well defined topologies has been investigated in polyacrylamide (PA) hydrogels prepared by copolymerization of acrylamide and __N, N__β²βmethylenebisacrylamide. Previous studies have reported structural and dynamic charac
Boundary element methods are used to model the free solution electrophoretic mobility of short DNA fragments. The Stern surfaces of the DNA fragments are modeled as plated cylinders that reproduce translational and rotational diffusion constants. The solventaccessible and ion-accessible surfaces are