A microfabricated hybrid device for DNA sequencing
โ Scribed by Shaorong Liu
- Publisher
- John Wiley and Sons
- Year
- 2003
- Tongue
- English
- Weight
- 391 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0173-0835
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## Abstract We present an approach for merging boundary element method (BEM) solutions of the electric field in electrically insulating complex geometries with Brownian dynamics (BD) simulations of DNA electrophoresis therein. Although a rote application of the standard BEM algorithm proves inaccur
Polyoma DNA was cleaved with restriction endonuclease HpaII, the fragments were separated by gel electrophoresis and transferred in good yield to separate nitrocellulose filters by a modification of the procedure of E. M. Southern (1975,J. Mol. Biol. 98,503-517.) The filters were then used in hybrid