A Micro-Bradford Membrane Protein Assay
β Scribed by Shu-Sheng Zuo; Per Lundahl
- Book ID
- 102563931
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 52 KB
- Volume
- 284
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
β¦ Synopsis
Tanzanians have a decreased capacity to metabolize omeprazole and mephenitoin in relation to their CYP2C19 genotype.
π SIMILAR VOLUMES
A procedure was developed for the quantitation of solubilized proteins using the Bradford assay in the presence of glucopyranoside detergents. These detergents solubilized membranebound proteins with minimal background absorbance at 595 nm. Absorbance at 650 nm was also low, indicating that these de
A technique for determining the amount of thermally denatured, insoluble protein is described. The assay has been validated using four globular proteins, bovine serum albumin, /Ilactoglobulin, lysozyme, and ovalbumin. It consists of a resolubilization protocol, using 8 M urea and 5% 2-mercaptoethano
Although the Bradford protein estimation assay has found wide distribution, it has a number of drawbacks, which in some cases have been shown to produce erroneous results upon comparison to other, more precise, methods for protein estimation. It was found that the underestimation of the protein cont