When protoplasts isolated from non-respiring rho Β° strains of S. cerevisiae are incubated with mitochondria from respiring rho + strains in the presence of polyethylene glycol, a small fraction of the acceptor protoplasts acquires respiratory capacity. Pretreatment of the isolated mitochondria and p
A Method of isolating Protoplasts from Yeast
β Scribed by EDDY, A. A.; WILLIAMSON, D. H.
- Book ID
- 109591698
- Publisher
- Nature Publishing Group
- Year
- 1957
- Tongue
- English
- Weight
- 286 KB
- Volume
- 179
- Category
- Article
- ISSN
- 0028-0836
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Mature leaves of Secale cereale cut into narrow strips and incubated for 18 h in a mixture of cellulase (Meicelase) and pectinase (Pectinol R10) produced quantities of protoplasts. Under the same conditions leaves of Triticum aestivum, Hordeum vulgare and Avena sativa also produce protoplasts but in