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A method of indirect mutant selection in Physarum polycephalum using the antibiotic netropsin

✍ Scribed by Gorman, Jessica A. ;Dove, William F.


Book ID
104726379
Publisher
Springer
Year
1974
Tongue
English
Weight
351 KB
Volume
133
Category
Article
ISSN
0026-8925

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A simple method for the physical separation of dormant and germinated cells of Phycornyces blakesleeanus has been designed. Separation was attained by isopycnic centrifugation in a preformed discontinuous density gradient of Urografin. In this gradient, dormant spores and active cells at two differe