๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

A method for the selection of mutants ofPhycomyces blakesleeanusdefective in germination

โœ Scribed by J. L. Micol; F. J. Murillo

Book ID
104759791
Publisher
Springer-Verlag
Year
1986
Tongue
English
Weight
474 KB
Volume
10
Category
Article
ISSN
0172-8083

No coin nor oath required. For personal study only.

โœฆ Synopsis

A simple method for the physical separation of dormant and germinated cells of Phycornyces blakesleeanus has been designed. Separation was attained by isopycnic centrifugation in a preformed discontinuous density gradient of Urografin. In this gradient, dormant spores and active cells at two different stages of germination were separated in three homogeneous and clearly distinct bands. The method has been used to follow the kinetics of the variation of cellular density during germination of wild type spores and to select for mutants temperature-sensitive for heat-shock induced germination. Sixty-nine mutants have been isolated by various procedures which include a separation step in the described Urografin gradient.

๐Ÿ“œ SIMILAR VOLUMES

Action spectra of the light-growth respo
Action spectra of the light-growth response in three behavioral mutants ofPhycomyces
โœ Peter A. Ensminger; Edward D. Lipson ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› Springer-Verlag ๐ŸŒ English โš– 478 KB

Null-point action spectra of the light-growth response were measured for three mutants of Phycomyces blakesleeanus (Burgeff) and compared with the action spectrum of the wild type (WT). The action spectrum for L150, a recently isolated "night-blind" mutant, differs from the WT spectrum. The L150 act