A method for the quantitative analysis of hydroxylysine and its glycosidic derivatives using high-voltage paper electrophoresis and fluorescamine

✍ Scribed by Charles Cintron; Benigno D. Peczon; Claire L. Kublin

• Publisher: Elsevier Science
• Year: 1978
• Tongue: English
• Weight: 819 KB
• Volume: 87
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(78)90713-3

✦ Synopsis

A new technique can measure nanomole levels of hydroxylysine, glucosylgalactosyl hydroxylysine (GGH), and galactosyl hydroxylysine (GH) as well or better than most amino acid analyzer methods. Amino acids are separated on high-voltage paper electrophoresis and treated with fluorescamine. On an equimolar basis, the relative fluorescence yield of GGH to hydroxylysine was 1.16, and that of GH was I .07. Proper standards must be run simultaneously with all samples to offset incomplete recovery of amino acids after separation and elution. The method is reproducible with hydroxylysine standards and amino acids from tissue samples. and several samples can be run at the same time.