A method for the determination of amyloid in plant seeds
β Scribed by P. Kooiman
- Publisher
- Elsevier Science
- Year
- 2010
- Tongue
- English
- Weight
- 191 KB
- Volume
- 79
- Category
- Article
- ISSN
- 0165-0513
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β¦ Synopsis
Abstract
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A colorimetric method has been elaborated for the determination of amyloids in plant seeds, based on the blue colour of the iodine complex in concentrated sodium sulphate solution.
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Details of the method are given and its possibilities and limitations are outlined.
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Three procedures for removing interfering starch are recommended.
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Methionine is present in small amounts in proteins, and methods for its determination usually involve acid hydrolysis of the protein and subsequent amino acid analysis (1) although there are a number of colorimetric methods available (2). However, methionine sulfur is easily oxidized, and it is poss