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A method for simultaneous detection of the transcripts of different cellular genes

✍ Scribed by Ze-Jin Liu; Ning-Hua Wu; Yu-Fei Shen

Book ID
118387986
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
237 KB
Volume
19
Category
Article
ISSN
0165-022X

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Optimal conditions for the detection of complex RNA sequences in individual cells by in situ hybridization have been determined by using in vitro cultured quail embryonic cells infected with Rous sarcoma virus and a single-stranded 3H-cDNA probe of high specific activity complementary to the RSV gen