A method for quantitative determination of cellular immunoglobulins by enzyme-labeled antibodies

✍ Scribed by S. Avrameas; Brigitte Guilbert

• Publisher: John Wiley and Sons
• Year: 1971
• Tongue: English
• Weight: 326 KB
• Volume: 1
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830010518

✦ Synopsis

this way. TKe visualization of antibodies after electrofocusing exploits the high sensitivity possible with radioactive antigens without the problems of non-specific binding which sets a limit to the lowest level of antibody detectable by conventional antigen binding tests [ 111. The potential of the present method for quantitation of antibodies remains to be explored. I thank Roger West for his valuable assistance and Dr. P. C. Montgomery for advice and criticism during development of the methods.