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A method for quantitating nanogram amounts of soluble protein using the principle of silver binding

✍ Scribed by Gerald Krystal; Chris Macdonald; Brad Munt; Stephen Ashwell

Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
754 KB
Volume
148
Category
Article
ISSN
0003-2697

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A dot-blot assay for quantitation of nan
A dot-blot assay for quantitation of nanogram amounts of protein in the presence of carrier ampholytes and other possibly interfering substances
✍ Martin Guttenberger; Volker Neuhoff; RΓΌdiger Hampp πŸ“‚ Article πŸ“… 1991 πŸ› Elsevier Science 🌐 English βš– 603 KB

A method for protein determination in one- and two-dimensional electrophoresis sample buffer is presented. Accurate quantitation of protein in two-dimensional electrophoresis sample buffer (9.5 M urea, 2% Nonidet P-40, 2% carrier ampholytes, and 5% 2-mercaptoethanol) required removal of carrier amph