A dot-blot assay for quantitation of nan
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Martin Guttenberger; Volker Neuhoff; RΓΌdiger Hampp
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Article
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1991
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Elsevier Science
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English
β 603 KB
A method for protein determination in one- and two-dimensional electrophoresis sample buffer is presented. Accurate quantitation of protein in two-dimensional electrophoresis sample buffer (9.5 M urea, 2% Nonidet P-40, 2% carrier ampholytes, and 5% 2-mercaptoethanol) required removal of carrier amph