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[3H]dansyl chloride: A useful reagent for the quantitation and molecular weight determination of nanogram amounts of protein

✍ Scribed by Richard M. Schultz; Paul M. Wassarman


Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
994 KB
Volume
77
Category
Article
ISSN
0003-2697

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A method for protein determination in one- and two-dimensional electrophoresis sample buffer is presented. Accurate quantitation of protein in two-dimensional electrophoresis sample buffer (9.5 M urea, 2% Nonidet P-40, 2% carrier ampholytes, and 5% 2-mercaptoethanol) required removal of carrier amph