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A method for preparing stable density gradients and their application for fractionation of intestinal mucosal cells

✍ Scribed by H. Huebers; E. Huebers; J. Simon; W. Forth


Book ID
118935297
Publisher
Elsevier Science
Year
1971
Tongue
English
Weight
268 KB
Volume
10
Category
Article
ISSN
0024-3205

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A method for fractionating rat liver cel
✍ Valerio M. Genta; David G. Kaufman; William K. Kaufmann πŸ“‚ Article πŸ“… 1975 πŸ› Elsevier Science 🌐 English βš– 661 KB

Sonically disrupted nuclei from proliferating liver cells were fractionated in Cs2S0, equilibrium density gradients. Nuclear constituents were concentrated in three bands designated as light band (LB, 1.21 g/cm"), middle band (MB, 1.26 g/cm"), and heavy band (HB, 1.32 g/cm"). Analysis of protein and