A method for preparing proteins and peptides for microsequencing
β Scribed by Willi Jahnen-Dechent; Richard J. Simpson
- Book ID
- 112821687
- Publisher
- Springer
- Year
- 1990
- Tongue
- English
- Weight
- 584 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0735-9640
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract Greatly reduced automated protein sequencing degradation times of less than 25 min with concurrent onβline phenylthiohydantoin (PTH) derivative analysis times of less than 16 min have been achieved by using a miniaturized reaction cartridge with optimized chemical and analytical cycles.
A new method has been developed for the isolation of proteins for microsequencing. Proteins were separated by isoelectric focusing on polyacrylamide slab gels. Ampholytes in the gel were washed out with 3.5% (v/v) perchloric acid, and the proteins were electroblotted onto unmodified glass-fiber shee