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A method for isolation and culture of lymphocytes from endoscopic biopsies

โœ Scribed by H.A. Brown; J. Douglas; C.B. Williams; J.A. Walker-Smith

Book ID
118898576
Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
418 KB
Volume
54
Category
Article
ISSN
0022-1759

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A technique is described for isolation and culture of adult human hepatocytes from surgical liver biopsies. The mean cell yield was 1.75 X 10(7) cells per gm liver and viability averaged 80%. Hepatocytes were maintained in primary culture for about 10 days. Cell morphology and histochemical characte