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A method for isolating nuclei from Euglena gracilis

✍ Scribed by Aprille, June R. ;Buetow, D. E.

Book ID: 104761847
Publisher: Springer-Verlag
Year: 1973
Weight: 625 KB
Volume: 89
Category: Article
ISSN: 0003-9276
DOI: 10.1007/bf00408902

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## Abstract We describe a rapid method whereby isolated chloroplasts from __Euglena gracilis__ can be separated in a Ficoll gradient according to their surface pK's by isoelectric focusing in one hour or less. The linear Ficoll gradient is made isotonic over the whole separation distance by additio