✦ LIBER ✦
Diversification of isoelectric points from isolated chloroplasts during the cell cycle of Euglena gracilis, strain z
✍ Scribed by Dr. Peter Brandt; Dorothee Ernst
- Publisher
- John Wiley and Sons
- Year
- 1982
- Tongue
- English
- Weight
- 181 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0173-0835
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✦ Synopsis
Abstract
We describe a rapid method whereby isolated chloroplasts from Euglena gracilis can be separated in a Ficoll gradient according to their surface pK's by isoelectric focusing in one hour or less. The linear Ficoll gradient is made isotonic over the whole separation distance by addition of sucrose. No released proteins of the isolated chloroplasts are detectable after isoelectric focusing. Modifications of the chloroplast envelope during the cell cycle of Euglena gracilis are evident by alterations of their isoelectric points.