A method for isolating individual cells from heterogeneous monolayer cultures
โ Scribed by Julie Morgan; Mark Levin; Stephen Bittner; Louis Cohen
- Book ID
- 105006368
- Publisher
- Springer
- Year
- 1976
- Tongue
- English
- Weight
- 218 KB
- Volume
- 2
- Category
- Article
- ISSN
- 1573-0603
No coin nor oath required. For personal study only.
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Cerebral blood vessels have unique properties when compared to most peripheral vascular beds. One such property is that large cerebral vessels are involved in the regulation of cerebral vascular resistance. Studying smooth muscle cells isolated from these vessels will determine how phenotypic proper
## A new technique is described which allows determination of solute uptake into cultured cell monolayers at times approaching 0.5 s. The apparatus consists of a section of a plastic tissue culture multidish which is held in a rotatable clamp and a manifold for simultaneous washing of the multidis