✦ LIBER ✦

A rapid sampling method for measuring uptake of small molecules by cultured cell monolayers

✍ Scribed by Robert S. Slaughter; Eugene M. Barnes Jr.

Publisher: Elsevier Science
Year: 1981
Tongue: English
Weight: 205 KB
Volume: 117
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(81)90692-8

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✦ Synopsis

A new technique

is described which allows determination of solute uptake into cultured cell monolayers at times approaching 0.5 s. The apparatus consists of a section of a plastic tissue culture multidish which is held in a rotatable clamp and a manifold for simultaneous washing of the multidishes with ice-cold buffer. This technique and apparatus was used to measure the initial rates of nucleoside and base transport by Chinese hamster fibroblasts.

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