For characterizing keto acids, paper chromatography of their DNPHa is most widely used irrespective of the disadvantages (1)) some of which are as follows: According to the methods reported before 1962, the DNPH formed by the usual method were extracted from water solution with an organic solvent su
A method for extraction and determination of 2,4-dinitrophenylhydrazones of keto acids
β Scribed by Chizoko Kawano; Hirohiko Katsuki; Tsuya Yoshida; Shozo Tanaka
- Publisher
- Elsevier Science
- Year
- 1962
- Tongue
- English
- Weight
- 436 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Thin-layer chromatography of keto acid 2,4-dinitrophenylhydrazones was carried out on plate coated with silica gel and sodium bicarbonate. A clear separation was obtained between most of the various keto acid hydrazones. The hydrazone was eluted from the plate with methanol containing 1% concentrate
A large number of reports have appeared for the determination of keto acids by the DNP\* method. The outline of the method is as follows: Keto acid in water solution is converted to DNPH derivative. Then the DNPH, after extraction with water-immiscible organic solvent, is transferred to water soluti
A rapid method for the analysis of pyrethrum extract and pyrethrum formulations containing synergists and emulsifying agents has been developed. The pyrethrins are converted into their 2,4-dinitrophenylhydrazones and, wwithout chromatography, the optical density at 377 mp determined. The optical den
Intracellular redox levels play an important role in physiology and pathophysiology. The principal intracellular reductant is NADPH, which is required for both the proper activity of the entire antioxidant system and important prooxidant enzymes such as nitric oxide synthase and NADPH oxidase. Thus