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A method for eliminating unnecessary serial thin sectioning for electron microscopy

✍ Scribed by Milroy, Antonia M.

Publisher
Wiley (John Wiley & Sons)
Year
1985
Tongue
English
Weight
122 KB
Volume
2
Category
Article
ISSN
0741-0581

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✦ Synopsis

a n Francisco, C A 94143 \ t!.ne ja,:ig -nethod for avoiding long serial ultra-thin sectioning has been developed by adding an ;:iter ncdiate step of 9 : r n semi-thin sections between the vibratome sectioning and t h e ultra-thin seitisn:ng (5ee figure 1). The a s e of t h e specimen advance upper limit of t h e ultramicrotome (LKB V) f ~r c.i?t.ng + J T serial semi-thin sections (block face approximately 1x2 m m ) and mounting them on

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## Abstract A method is described for the preparation of cross‐sectional samples of thin films for transmission electron microscopy. The technique produces larger amounts of thin region as compared with ion milling and eliminates the problems associated with ion beam damage. The requirement is that