๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

A method for direct quantification of the amount of DTPA in 111In monoclonal antibody preparations

โœ Scribed by T.S.T. Wang; A.K. Ng; S. Alsedairy; R.A. Fawwaz; M.A. Hardy; P.O. Alderson

Publisher
Elsevier Science
Year
1987
Weight
995 KB
Volume
38
Category
Article
ISSN
0883-2889

No coin nor oath required. For personal study only.

โœฆ Synopsis

The bicyclic anhydride of DTPA-1-14C (BADTPA-1-14C) was synthesized and reacted with an antibody to human melanoma associated antigen (MAA) and with one to human class II major histocompatibility complex antigen (HLA-DR). DTPA-1-C incorporation per mole of anti MAA at molar ratios of 1:1 to 200:1 ranged from 0.5 to 25, while immunoreactivity ranged from 49 to 9%. With antibody to HLA-DR, results were similar. Anti MAA, but not anti HLA-DR, demonstrated polymerization upon conjugation. BADTPA-1-14C provides a convenient and accurate method for measuring the amount of DTPA in monoclonal antibody preparations and its effect on immunoreactivity.

๐Ÿ“œ SIMILAR VOLUMES

A sensitive immunoradiometric assay for
A sensitive immunoradiometric assay for the quantification of murine monoclonal antibodies in human serum
โœ John M. Apathy; Ronald R. Bowsher ๐Ÿ“‚ Article ๐Ÿ“… 1989 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 578 KB

The clinical investigation of murine monoclonal antibodies (MoAbs) as potential immunotherapeutic agents necessitates their quantification in human serum. The present paper reports the development of a sensitive, non-competitive "sandwich" immunoradiometric assay specifically optimized for measureme

Radiopharmaceutical preparation of a mon
Radiopharmaceutical preparation of a monoclonal antibody, lym-1, and its F(ab')2 fragment for imaging lymphoma with In-111
โœ Stanley L. Mills; Sally J. Denardo; Gerald L. DeNardo; Shrikant V. Deshpande; Mi ๐Ÿ“‚ Article ๐Ÿ“… 1989 ๐Ÿ› John Wiley and Sons ๐ŸŒ French โš– 500 KB

Lym-1) or its fragment F(ab')2 to produce two new radiopharmaceuticals. Immunoreactivity of the Lym-1, specific for B cell lymphoma, was not affected by the coupling reaction for 1.0 and 4.3 CITC molecules per whole antibody and 3.0 CITC molecules per F(ab')2 fragment. Both retained in excess of 90%