Radiopharmaceutical preparation of a monoclonal antibody, lym-1, and its F(ab')2 fragment for imaging lymphoma with In-111

✍ Scribed by Stanley L. Mills; Sally J. Denardo; Gerald L. DeNardo; Shrikant V. Deshpande; Michael McCall; Claude Meares; Alan L. Epstein

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: French
• Weight: 500 KB
• Volume: 27
• Category: Article
• ISSN: 0022-2135
• DOI: 10.1002/jlcr.2580270403

✦ Synopsis

Lym-1) or its fragment F(ab')2 to produce two new radiopharmaceuticals. Immunoreactivity of the Lym-1, specific for B cell lymphoma, was not affected by the coupling reaction for 1.0 and 4.3 CITC molecules per whole antibody and 3.0 CITC molecules per F(ab')2 fragment. Both retained in excess of 90% immunoreactivity even at the level of 25 mCi of In-111 per mg of Lym-1 and 15 mCi per m F(Ab')2. The labeling reaction produced yields in excess of 9 8 4 eliminating the requirement for routine purification of the final radiopharmaceutical.