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A method for determinations of microgram amounts of chitin in arthropod cuticles

โœ Scribed by R.H. Hackman; Mary Goldberg

Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
359 KB
Volume
110
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A method is described for determining microgram amounts of chitin in arthropod cuticles. The method, adapted from one described for assaying plant material for fungal content, is based on estimating soluble chitosan derivatives and is not affected by the presence of nonchitinous cuticular components. There is good agreement between the results given by the micromethod and those given by a semimicro method based on material insoluble in hot, dilute alkali. As a qualitative test for chitin the limit for the method is Cl00 ng.

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