A simple turbidimetric method for the determination of microgram quantities of Trition X-100

✍ Scribed by Kazunari Yoshida; Kazuhiko Ishihara; Susumu Ohara; Yoshiteru Azuumi; Kyoko Hotta

• Publisher: Elsevier Science
• Year: 1980
• Tongue: English
• Weight: 276 KB
• Volume: 108
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(80)90707-1

✦ Synopsis

A simple and sensitive procedure for the quantitative estimation of Triton X-100 is described. The method is based on the formation of turbidity from Triton X-100 with phenol. The turbidity is proportional to Triton X-100 in a range of 20-80 &ml. Protein, mucopolysaccharide, and nucleic acid do not interfere in this turbidity formation. The method is especially useful for detection of the residue after the removal of Triton X-100 from solubilized samples.