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A method for determination of glucose 1,6-bisphosphatase

✍ Scribed by A.M. Bassols; J. Carreras; R. Cussó


Book ID
118387933
Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
330 KB
Volume
16
Category
Article
ISSN
0165-022X

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A sensitive and rapid spectrophotometric method for determination of glucose-6-phosphatase activity is described. Glucose formed by the enzyme is oxidized by glucose oxidase to gluconolactone and hydrogen peroxide. The latter, phenol, and 4-aminoantipyrine are converted by peroxidase to quinoneimine