A method for determination of 3,4-dihydroxyphenylacetic acid and 3,4-dihydroxymandelic acid in urine
✍ Scribed by B.D. Drujan; N. Alvarez; J.M. Díaz Borges
- Publisher
- Elsevier Science
- Year
- 1966
- Tongue
- English
- Weight
- 506 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0003-2697
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A continuous spectrophotometric method for the rapid determination of diphenolase activity of tyrosinase is described. It uses 3,4-dihydroxymandelic acid (DOMA) as the substrate of tyrosinase and measures the final product, 3,4-dihydroxybenzaldehyde (DOBA). The spectrum of this product shows a batho
Specifically labelled phenylacetic acid and mandelic acid derivatives, metabolites of L-Dopa, have been synthesized via the intermediary labelled benzyl alcohols, which were prepared by reduction of the methyl esters of the appropriate benzoic acids. The benzyl alcohols have been converted to the co