β Scribed by Bernd Lammel
No coin nor oath required. For personal study only.
A method for continuous preparative electrophoresis is presented. It is based on the principle that a supporting medium, e.g. a polyacrylamide gel, is moved continuously relative to an electrical field. At one fixed point, the sample is applied to the gel. The charged particles migrate with different velocities in the supporting medium. Simultaneously, they are dragged along with the supporting medium. This procedure leads to a twoβdimensional separation of charged particles, which is necessary for a continuous operation of an electrophoresis apparatus. Three different designs of apparatuses, using this method, are presented in this paper. The operation of one construction was tested by separating three protein mixtures.
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