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A device for preparative elution electrophoresis using a polyacrylamide gel slab

✍ Scribed by Helen C. Carpenter; Dr. John H. Skerritt; Colin W. Wrigley; Joel Margolis

Publisher: John Wiley and Sons
Year: 1986
Tongue: English
Weight: 653 KB
Volume: 7
Category: Article
ISSN: 0173-0835
DOI: 10.1002/elps.1150070507

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✦ Synopsis

A device for preparative elution electrophoresis using a polyacrylamide gel slab

A compact device, capable of being clipped onto the end of a 3 mm thick analytical polyacrylamide slab gel, has been developed for the collection of resolved protein zones as they elute from the end of the gel. Its unique feature is that eluted proteins were collected between two polyacrylamide-embedded paper membranes and removed either by continuous or intermittent buffer elution. Because of this direct coupling to large gels identical to those used for analytical electrophoresis, protein zones were obtained with high resolution. The method was applicable to non-denaturing cathodic and anodic electrophoresis, as well as sodium dodecyl sulfate electrophoresis. Medium-scale quantities (up to 10 mg/cm2 cross-sectional area) ofprotein were fractionated in as little as 4 h with high recoveries.

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