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A method for changing the pH of gel strips in the two-dimensional gel electrophoresis of ribosomal proteins

✍ Scribed by S. Avital; D. Elson

Book ID
107711538
Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
148 KB
Volume
57
Category
Article
ISSN
0003-2697

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Characterisation of the ribosomal protei
Characterisation of the ribosomal proteins from Schizosaccharomyces pombe by two-dimensional polyacrylamide gel electrophoresis
✍ Coddington, Alan ;Fluri, Rudolf πŸ“‚ Article πŸ“… 1977 πŸ› Springer 🌐 English βš– 740 KB

The 80S ribosome of Schizosaccharomyces pombe contains 93 proteins as determined by twodimensional electrophoresis on polyacrylamide gels. Of these, 76 are basic and 17 are acidic at pH 8.7. 38 proteins could be assigned unambiguously to the large sub-unit and 19 to the small. 67 proteins were extra