A low-cost polyacrylamide gel scanning densitometer

✍ Scribed by David K. Shortess

• Publisher: Elsevier Science
• Year: 1979
• Tongue: English
• Weight: 364 KB
• Volume: 100
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(79)90103-9

✦ Synopsis

A simple, low-cost, home-built acrylamide gel scanning densitometer is described. The instrument was designed to provide gel scans for the purpose of comparing the relative rates of mobility of bands. It does not include provisions for large volume, integration of peaks, or micrometer resolution. The key to the simplicity of the device was through the use of a rotary rather than a transverse gel carriage. A turntable was attached directly to the shaft of a synchronous clock motor with a slow rate of rotation. A curved gel holder, placed on the turntable, carries the gel through the light beam. A commercial strip chart recorder is used with the scanner.