A liquid chromatographic method for the determination of danazol in human serum

✍ Scribed by Krzysztof Selinger; Howard M. Hill; June A. Anslow; Diana Gash

• Publisher: Elsevier Science
• Year: 1990
• Tongue: English
• Weight: 439 KB
• Volume: 8
• Category: Article
• ISSN: 0731-7085
• DOI: 10.1016/0731-7085(90)80010-m

✦ Synopsis

A liquid chromatographic method for the determination of danazol in human serum has been developed. Reversed-phase C8 and C18 columns were used with a column-switching valve, isocratic elution and UV detection. Sample pretreatment involved extraction of the drug with pentane-methylene chloride. The method enabled the measurement of the drug at a concentration as low as 1 ng ml-1, with precision of 15.0% and accuracy of 8.3%. The method was used to run a two way replicated pharmacokinetic study of danazol. The main pharmacokinetic parameters were (mean of two periods): AUCinf = 480.94 ng x h ml-1, Cmax = 53.2 ng ml-1, tmax = 2.5 h, t0.5 = 18.00 h.