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A laboratory-intensive course on the experimental study of protein–protein interactions

✍ Scribed by D.Scott Witherow; Sue Carson

Publisher
The American Society for Biochemistry and Molecular Biology
Year
2011
Tongue
English
Weight
379 KB
Volume
39
Category
Article
ISSN
1470-8175

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The study of protein–protein interactions is important to scientists in a wide range of disciplines. We present here the assessment of a lab‐intensive course that teaches students techniques used to identify and further study protein–protein interactions. One of the unique elements of the course is that students perform a yeast two‐hybrid screen and identify novel protein–protein interactions in what is essentially the beginning of an independent research project in the context of a class. While students benefit from the research‐like experience, data is actively generated that can be further studied in independent research projects. Student learning outcomes were assessed using a questionnaire that was given to students before and after the course. The results indicate that students' conceptual and technical understanding of the methodologies taught in the class increased, and that hands‐on experience in the lab was perceived to be the most important component of the course. Biochemistry and Molecular Biology Education Vol. 39, No. 4, pp. 300‐308, 2011

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