A spectrophotometric method for the dete
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P. Gierow; B. Jergil
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Article
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1980
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Elsevier Science
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English
β 361 KB
A sensitive and rapid spectrophotometric method for determination of glucose-6-phosphatase activity is described. Glucose formed by the enzyme is oxidized by glucose oxidase to gluconolactone and hydrogen peroxide. The latter, phenol, and 4-aminoantipyrine are converted by peroxidase to quinoneimine