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A kinetic-spectrophotometric method for the determination of glucose in solutions

✍ Scribed by M. A. Zaitoun


Book ID
110211610
Publisher
SP MAIK Nauka/Interperiodica
Year
2006
Tongue
English
Weight
158 KB
Volume
61
Category
Article
ISSN
1061-9348

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A sensitive and rapid spectrophotometric method for determination of glucose-6-phosphatase activity is described. Glucose formed by the enzyme is oxidized by glucose oxidase to gluconolactone and hydrogen peroxide. The latter, phenol, and 4-aminoantipyrine are converted by peroxidase to quinoneimine