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A highly specific and sensitive determination of gamma-aminobutyric acid by gas chromatography mass spectrometry

✍ Scribed by B. N. Colby; M. W. McCaman

Publisher
John Wiley and Sons
Year
1978
Tongue
English
Weight
508 KB
Volume
5
Category
Article
ISSN
1076-5174

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✦ Synopsis

A procedure for the identification and quantification of picomole quantities of gamma-aminobutyric acid in tissue samples is given. This procedure combines the chemical specificity of dinitrophenylation with that of gas chromatography mass spectrometry to eliminate the interferences encountered with other direct derivatization procedures. Only a limited number of dinitrophenyl amino acid ethyl esters and some fatty ethyl esters are detected in the solution used for analysis. Identification is based on retention time and on the relative abundances of the three major ion fragments of the gamma-aminobutyric acid derivative. Quantitation is accomplished using isotope dilution techniques with [2H2]gamma-aminobutyric acid as an internal standard. The procedure has been successfully applied to samples of human cerebrospinal fluid and to extracts of ganglia from the mollusc, Aplysia californica.

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