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A highly sensitive and selective homogenous assay for profiling microRNA expression

โœ Scribed by Deng, Huimin; Shen, Wei; Ren, Yuqian; Gao, Zhiqiang

Book ID
122242505
Publisher
Elsevier Science
Year
2014
Tongue
English
Weight
781 KB
Volume
54
Category
Article
ISSN
0956-5663

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A new method for the measurement of poly(ADP-ribose) is described. Poly(ADP-ribose) was separated from the bulk of cellular RNA and DNA by quantitative adsorption to dihydroxyboryl-Sepharose. The polymer was digested with venom phosphodiesterase and bacterial alkaline phosphatase to yield the uniqu