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A high-throughput DNA extraction method for barley seed

โœ Scribed by Rebecka von Post; Lars von Post; Christophe Dayteg; Marie Nilsson; Brian P. Forster; Stine Tuvesson


Book ID
110426603
Publisher
Springer
Year
2003
Tongue
English
Weight
147 KB
Volume
130
Category
Article
ISSN
0014-2336

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Genome projects require a high-throughput method for DNA template preparation, which traditional protocols can rarely provide. We introduce here a new protocol for plasmid and cosmid DNA preparation based on alkaline denaturation in a 96 -well format. The essential improvement made in this protocol