✦ LIBER ✦

A high recovery method for concentrating microgram quantities of protein from large volumes of solution

✍ Scribed by D. Mahuran; P. Clements; M. Carrella; P.M. Strasberg

Publisher: Elsevier Science
Year: 1983
Tongue: English
Weight: 611 KB
Volume: 129
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(83)90585-7

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✦ Synopsis

A method is presented for the recovery of 40-80% of the protein from a I ~gjml solution. The final protein pellet is free of detergent and other ionic compounds and is thus compatible with any denaturing solution. The primary structure of the protein is unaffected by the procedure, making the final pellet an ideal sample for any analytical procedure to determine protein structure.

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A rapid method of concentrating proteins in small volumes with high recovery using sephadex G-25

✍ Allan Saul; Michele Don 📂 Article 📅 1984 🏛 Elsevier Science 🌐 English ⚖ 279 KB

A method for concentrating small (10 to 500 microliter) volumes of protein solutions by mixing in a microcentrifuge tube with measured volumes of dry Sephadex G-25 is described. The tube is centrifuged, and the protein solution concentrated up to fivefold passes through a small hole in the base of t