✦ LIBER ✦
A rapid method of concentrating proteins in small volumes with high recovery using sephadex G-25
✍ Scribed by Allan Saul; Michele Don
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 279 KB
- Volume
- 138
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A method for concentrating small (10 to 500 microliter) volumes of protein solutions by mixing in a microcentrifuge tube with measured volumes of dry Sephadex G-25 is described. The tube is centrifuged, and the protein solution concentrated up to fivefold passes through a small hole in the base of the tube and is collected. Recovery of protein is 90-95%.