Pyruvate kinase catalyzes the conversion of phosphoenolpyruvate (PEP) to pyruvate. A direct radioassay for this enzyme using [14C]PEP as substrate has been developed. The product, [14C]pyruvate, can be separated from the substrate rapidly and easily by applying the mixture to a hydroxyapatite column
A hexokinase-coupled radioassay for pyruvate kinase
β Scribed by Ana Preller; Tito Ureta
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 361 KB
- Volume
- 76
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
Pyruvate kinase (PK) (EC 2.7.1.40, ATP:pyruvate phosphotransferase) is one of the two enzymes in glycolysis involved in substrate phosphorylation. It catalyzes the following reaction: phosphoenolpyruvate + ADP -+ pyruvate i-ATP (-4)
In this study, it is found that, for Bacillus subtilis, citrate-glucose cometabolism leads to zero acid pro- duction over a wide range of growth rates and nearly theoretical carbon yield. Experimental results are presented that point to pyruvate kinase (PYK) as a site of citrate-mediated glycolytic
## Abstract Proliferating cells, particularly the tumor cells, express a dimeric isoenzyme of pyruvate kinase, termed M2βPK. It's a direct target of several oncoproteins; the determination of fecal tumor pyruvate kinase type M2 (M2βPK) might be another promising tool for colorectal cancer (CRC) scr