Pyruvate kinase catalyzes the conversion of phosphoenolpyruvate (PEP) to pyruvate. A direct radioassay for this enzyme using [14C]PEP as substrate has been developed. The product, [14C]pyruvate, can be separated from the substrate rapidly and easily by applying the mixture to a hydroxyapatite column
A direct spectrophotometric assay for pyruvate kinase
โ Scribed by Ning G. Pon; Richard J.L. Bondar
- Publisher
- Elsevier Science
- Year
- 1967
- Tongue
- English
- Weight
- 433 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0003-2697
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โฆ Synopsis
Pyruvate kinase (PK) (EC 2.7.1.40, ATP:pyruvate phosphotransferase) is one of the two enzymes in glycolysis involved in substrate phosphorylation. It catalyzes the following reaction: phosphoenolpyruvate + ADP -+ pyruvate i-ATP (-4)
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